Technique / Molecular Biology / Bacterial plasmid bacterial phages vectors


Glycerol stock longevity



Glycerol stock longevity

Does any one have information (data or opinion or personal experience) on
how
long a bacterial glycerol stock is viable @ -80oC? How often to stocks fail
to
regrow? Is is necessary to thaw and revitalize the stock at time intervals?

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Last update 12-May-2002, Rating of 2 votes.

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By Ashok yadav on 11-Feb-2007
why glycerol stock is not used repeatedily
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By Mayank Rai on 27-Jul-2006
I suspect that you did not elute the the 146bp band from the gel after the PCR reaction, and directly used the PCR cocktail or PCR purified product for A-tailing and/or ligation. You must be having some 800bp amplified fragment in your PCR mix which was not removed. so elute fragment from the gel.
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By creageng on 12-May-2002
My experiment have a puzzled trouble.Can any one kindly help me.

My target gene is 146bp in length.But when I got two colone(from total about 80 colones using promega easy T-vector),it seems that the colone contain 800bp insert.what caused that matter.could you tell me.pls.thank you
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