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Molecular Cloning: A Laboratory Manual



Molecular Cloning: A Laboratory Manual

Designed to be used in tandem with the Web site, this three-volume set provides complete descriptions of 250 laboratory protocols in DNA science--35 percent of which were created especially for this edition.



Molecular Cloning: A Laboratory Manual

Coverage includes techniques for isolating, analyzing and cloning both large and small DNA molecules; cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acids probes, mutagenesis, and DNA sequencing; and methods to screen expression libraries, express cloned genes in both pro- and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. In addition to annotations within protocols, 115 information panels spread throughout the volumes provide insight into the reasons why methods are carried out in a certain manner and how techniques have evolved. The appendices are a compendium of reagents, vectors, media, techniques, suppliers, kits, and electronic resources.

Last update 28-Jun-2007, Rating Excellent! of 4 votes.


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