Molecular Cloning: A Laboratory Manual

Designed to be used in tandem with the Web site, this three-volume set provides complete descriptions of 250 laboratory protocols in DNA science--35 percent of which were created especially for this edition.



Molecular Cloning: A Laboratory Manual

Coverage includes techniques for isolating, analyzing and cloning both large and small DNA molecules; cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acids probes, mutagenesis, and DNA sequencing; and methods to screen expression libraries, express cloned genes in both pro- and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. In addition to annotations within protocols, 115 information panels spread throughout the volumes provide insight into the reasons why methods are carried out in a certain manner and how techniques have evolved. The appendices are a compendium of reagents, vectors, media, techniques, suppliers, kits, and electronic resources.

Last update 28-Jun-2007, Rating of 4 votes.

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	By anil on 28-Aug-2007 i am a PG holder in biology plse let me know the baiscs of data base mangement in bioinofrmatics. Rating: Reply

	By surya Bhushan Kumar on 27-Aug-2007 iam mol bio exp. working at Biopharmaceuticals plz give me protocol of RT-PCR PCR WB Elisa and mab development Rating: Reply

	By Patel Rajesh kumar Manilal on 31-Jul-2007 This is very excellent book for Ph.D. students.sir I am a Ph.D. scholar in S.K.Patel Institute of Pharmacy, Ganpat University, India . Sir i am not economically sound to buy this book .If you send me this book via mail . Pls help me as consider as your child . Rajesh M.Patel Rating: Reply

	By santosh nagwani on 28-Jun-2007 Respected sir This is very excellent book for Ph.D. students.sir I am a Ph.D. scholar in IMS BHU , India . Sir i am not economically sound to buy this book .If you send me this book via mail . Pls help me as consider as your child . santosh nagwani Rating: Reply

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