E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology)

E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology (Clifton, N.J.), V. 235.)



Table of Contents

The Function and Organization of Plasmids
Finbarr Hayes

Choosing a Cloning Vector
Andrew Preston

Escherichia coli Host Strains
Nicola Casali

Chemical Transformation of E. coli
W. Edward Swords

Electroporation of E. coli
Claire A. Woodall

DNA Transfer by Bacterial Conjugation
Claire A. Woodall

Cosmid Packaging and Infection of E. coli
Mallory J. A. White and Wade A. Nichols

Isolation of Plasmids from E. coli by Alkaline Lysis
Sabine Ehrt and Dirk Schnappinger

Isolation of Plasmids from E. coli by Boiling Lysis
Sabine Ehrt and Dirk Schnappinger

High-Purity Plasmid Isolation Using Silica Oxide
Stefan Grimm and Frank Vo?Neudecker

High-Throughput Plasmid Extraction Using Microtiter Plates
Michael A. Quail

Isolation of Cosmid and BAC DNA from E. coli
Daniel Sinnett and Alexandre Montpetit

Preparation of Single-Stranded DNA from Phagemid Vectors
W. Edward Swords

Using Desktop Cloning Software to Plan, Track, and Evaluate Cloning Projects
Robert H. Gross

Cloning in Plasmid Vectors
Carey Pashley and Sharon Kendall

Extraction of DNA from Agarose Gels
Nicholas Downey

Cloning PCR Products with T-Vectors
Wade A. Nichols

Construction of Genomic Libraries in l-Vectors
Yilun Wang, Zheng Cao, Darryl Hood, and James G. Townsel

Rapid Screening of Recombinant Plasmids
Sangwei Lu

Restriction Analysis of Recombinant Plasmids
Joanne Goranson-Siekierke and Jarrod L. Erbe

Screening Recombinant DNA Libraries
Wade A. Nichols

Sequencing Using Fluorescent-Labeled Nucleotides
Allison F. Gillaspy

Site-Directed Mutagenesis Using the Megaprimer Method
Zhidong Xu, Alessia Colosimo, and Dieter C. Gruenert

Site-Directed Mutagenesis by Inverse PCR
Clifford N. Dominy and David W. Andrews

Creating Nested DNA Deletions Using Exonuclease III
Rosamund Powles and Lafras M. Steyn

Transposon and Transposome Mutagenesis of Plasmids, Cosmids, and BACs
Alistair McGregor

In Vitro Transcription and Translation
Farahnaz Movahedzadeh, Susana González Rico, and Robert A. Cox

Vectors for the Expression of Recombinant Proteins in E. coli
Sally A. Cantrell

Expression of Recombinant Proteins from lac Promoters
Charles R. Sweet

Plasmid-Based Reporter Genes: Assays for b-Galactosidase and Alkaline Phosphatase Activities
Minghsun Liu

Plasmid-Based Reporter Genes: Assays for Green Fluorescent Protein
Sergei R. Doulatov

Index

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Last update 15-Dec-2004, Rating Very Good of 3 votes.