Technique / Cell Biology / Cell culture / Cell preservation
Freezing Cells
Freezing Cells
Does any of you have any experience in freezing (and storing) single
cells at -20 dgr.C ?
What happens if a cell suspension (with DMSO) goes trough a temperature
profile of -10 to -70 degr.C?
Do you know of any references
... ...
Click following links for full story
Freezing
Cells
Last update 08-Jan-2001, Rating of 1 votes.
Post your message in Cell preservation forum
Write your comment
|
By lina
on 06-Dec-2005
do cells expand when frozen? |
|
|
By pushpa
on 02-May-2003
I put them in culture media with 10% DMSO,-80 |
|
|
By loubna
on 09-Jul-2002
To freeze cells, I put them in 90% heat inactivated calf serum and 10% DMSO.and directly at -20 for 2 hours, at -80 overnight and then in liquid nitrogen for unlimited time. |
|
|
By lut
on 08-Jan-2001
There is a quite good reference about Asymptote Cool Guide to Cryopreservation. You can read it online at http://www.asymptote.co.uk/manual/effects.htm. |
Please Login or Register to Post
