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Lambda protocol



Lambda protocol

Protocol from Rob Phillips Group.
The well-known lambda bacteriophage grows on E. coli bacteria. Here's what you need to know to work with it.

Preparation of plates with rich medium
Preparation of liquid medium
Titering lambda
TM buffer for storage
Generating different sizes of lambda with ZAP and puc19
Purifying lamB, the lambda receptor
Propagating and purifying lambda phage
Extracting lambda DNA and testing on a gel

Last update 31-Oct-2005, Rating of 4 votes.

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By tarbez nache on 30-May-2007
bonjour, je travaille dans un laboratoire de biologie moleculaire, j'ai besoin de connaitre les réferences les plus utiles pour travailler sur les levures et bacteries.
concernant les restricitions, le séquancage, la PCr ...
merci d'avance pour votre aide
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By Vyas Rohan.A on 19-Sep-2006
hi , my self rohan, i m doin MSc -2 microbiology, i m working with bacteriophages but i havent general protocol for isolation of phages. i need it because it is important for my project.
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By abhijit on 08-Apr-2006
i get good info. from this site .send me other info. about plasmid vectors

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By Diana Carolina Parra Chavez on 31-Oct-2005
Hi. I'm a biology's student. I'm working with bacteriophages, but I' don't have a general protocol for isolation of phages. I need it because it's important for my career's project.
Thanks.
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