The Joy of Cloning

A listing of the protocols that are generally used in the Schimenti Lab. They are extracted from our lab techniques manual, otherwise known as The Joy of Cloning.

Table of Contents
Purification of DNA
Phenol Extraction
Ethanol Precipitation
Purification of DNA from Gels
Low melting temperature agarose, standard method
Agarase Extraction of DNA Fragments
Isolation of DNA from Acrylamide Gels
DNA Isolation
From Tissues
Isolation of DNA from Tissues
Mouse Tail DNA Prep (the classic)
Mouse Brain DNA Prep for Southern Blotting
Genotyping Mice by PCR, I
Genotyping Mice by PCR,II
Genotyping Mice by PCR,III
We now use the HotSHOT method...by far the best: Biotechniques 29:52 (2000)

From bacteria (plasmids, cosmids)
Large Scale Preparation of Plasmid DNA by Alkaline Lysis
Mini Plasmid Prep
Recombinant DNA Techniques and Cloning
Spin Column chromatography
Southern Blotting
Oligo labeling of DNA probes
Colony Lifts and Screening, Standard and Autoclaving Methods
RNA WORK
acid phenol method
RNA Formaldehyde for NORTHERN Blotting
ES Cell Work
Trypsinizing Cells
DNA Extraction, Standard Method
Electroporation
Picking ES cell colonies after electroporation
Splitting and Freezing
96-well Plate Microextraction Protocol
Genotyping of colonies right after picking

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Last update 26-Nov-2004, Rating n/a of 0 votes.