RNA Silencing Methods and Protocols

RNA Silencing Methods and Protocols - by Carmichael, Gordon (University of Connecticut Health Center, Farmington, CT)

Human press publication. Link to Amazon.com. RNA Silencing: Methods and Protocols


Table of Contents


1. Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method
Mikiko C. Siomi and Haruhiko Siomi

2. Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis
John W. Pham and Erik J. Sontheimer

3. Preparation and Analysis of Drosha
Yoontae Lee and V. Narry Kim

4. RNA Interference in C. elegans
Nathaniel R. Dudley and Bob Goldstein

5. Down-Regulating Gene Expression by RNA Interference in Trypanosoma brucei
Christine E. Clayton, Antonio M. Estévez, Claudia Hartmann, Vincent P. Alibu, Mark Field, and David Horn

6. Analysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene Silencing
M. Florian Mette, Werner Aufsatz, Tatsuo Kanno, Lucia Daxinger, Philipp Rovina, Marjori Matzke, and Antonius J. M. Matzke

7. Strategies for the Design of Random siRNA Libraries and the Selection of Anti-GFP siRNAs
Mouldy Sioud and Sebastien Wälchli

8. Silencing Gene Expression with Dicer-Generated siRNA Pools
Jason W. Myers and James E. Ferrell, Jr.

9. Inhibition of Gene Expression In Vivo Using Multiplex siRNA
Sung-Suk Chae and Timothy Hla

10. Gene Silencing by a DNA Vector-Based RNAi Technology
Guangchao Sui and Yang Shi

11. Plasmid-Based RNA Interference: Construction of Small-Hairpin RNA Expression Vectors
Scott Q. Harper and Beverly L. Davidson

12. siRNA Delivery In Vivo
Mouldy Sioud

13. Peptide-Based Strategy for siRNA Delivery into Mammalian Cells
Federica Simeoni, May C. Morris, Frederic Heitz, and Gilles Divita

14. Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells
Mingjie Li and John J. Rossi

15. Exon-Specific RNA Interference: A Tool to Determine the Functional Relevance of Proteins Encoded by Alternatively Spliced mRNAs
Alicia M. Celotto, Joo-Won Lee, and Brenton R. Graveley

16. Immunoprecipitation of MicroRNPs and Cirectional Cloning of MicroRNAs
Elsa Maniataki, Maria Dels Angels De Planell Saguer, and Zissimos Mourelatos

17. Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro
Marianthi Kiriakidou, Peter Nelson, Stella Lamprinaki, Anup Sharma, and Zissimos Mourelatos

18. High Throughput Analysis of MicroRNA Gene Expression Using Sensitive cDNA Probes
Mouldy Sioud and Øystein Røsok

19. Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA
Peng Liu, Mary L. Stover, Alexander Lichtler, and David W. Rowe

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Last update 23-Dec-2005, Rating Very Good of 2 votes.