DNA extraction and DNA purification methods
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DNA extraction and DNA purification methods
Generally speaking, strategies for DNA extraction and DNA purification depends on your downstream applications, e.g. southern blot hybridization using high molecular weight genomic DNA, DNA fragments for subcloning, or DNA for PCR etc. Some considerations for DNA extraction and DNA purification:
1. Avoid nuclease contamination (DNase contamination).
2. Avoid shearing of intact DNA molecules.
3. Avoid chemical contamination that can interfere with downstream applications.
Common steps for DNA extraction and DNA purification:
1. Cell or tissue lysis containing detergents (SDS, triton X-100 etc), proteinase K, RNase etc.
2. DNA extraction step (avoid protein, RNA and chemical contamination).
3. DNA precipitation using 75-80% ethanol, isopropanol, PEG or TCA.
Some commonly used DNA extraction and DNA purification methods:
1. Salting out and DNA Precipitation (Guanidinium isothiocyanate).
2. Organic Solvents DNA extraction and DNA precipitation (phenol, chloroform).
3. Silica Resin-Based DNA purification.
4. Anion Exchange-based DNA purification
Last update 31-Dec-2006, Rating Good of 9 votes.
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I need to kow how DNA purification for preparative DNA probes. DNA from hepatocarcinogenesis tissue.
! will agree if you have some techiques.
Ph. D. Graciela Pérez Villaseñor
Universidad Autonoma Metropolitana. México.
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I wish to know more information about the PUREGENE method of DNA extraction. Any help would be appreciated.
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DNA extraction consist of 3 basic steps,which is homogenization,deproteinization and precipitation. can u elaborate the 3 steps?
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Indeed this is wonderful, please I need protocol for DNA extraction, purification and characterisation. Thanks
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After adding the solution2 you should not stand for more than 2min or the DNA will damege!!
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i am doin dna extraction with phenol chloroform method but shearing of dna is there . how can i aviod that? what are the critical steps, one should take care of?
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excellent i want protocol for dna quantificationa and for purifying the same
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dear Sir
can u give me DNA extraction and DNA purification methods
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all related procedure and protocols to RT-PCR used in viral detection.
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