Technique / Cell Biology / Cell cycle analysis
Cell synchronization protocol
Cell synchronization protocol
Detailed PDF protocol link to LabVelocity.com. Source from BioSource International.
In order to study many of the control mechanisms involved in cell cycle regulation,
it is critical to synchronize the cells so that the distinct phases of the cycle are being
studied. Synchronizing cells allows the precise study of regulatory mechanisms
which determine cell cycle regulation at the level of gene expression and protein
phosphorylation, facilitating drug discovery.
To synchronize cells, several methods are used. Commonly, researchers induce cells
to quiescence by serum starvation before releasing them from this state, or treat cells
with chemical inhibitors which arrest cells in distinct phases of the cycle. The following
is a brief summary of these methods
Last update 16-Jan-2008, Rating of 43 votes.
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By peng wang
on 07-Mar-2009
I am doing the oncogene processing cell cycle research. Would you send me the synchonizing protocol. Thanks very much! |
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By Maggie
on 04-Mar-2009
Is there any chance you could send me the protocols for cell synchronisation? Serum starvation to synchronise the cancer cells I am using would take forever and a day... |
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By Anand kumar
on 26-Feb-2009
I am interested in synchronization(S,G1&G2) and cell cycle. I'd like to recieve a protocol. |
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By VIJAY BARATHI
on 17-Feb-2009
Thymidine is commonly used to synchronise cells.Nocodazole is added in addition to prevent the polymerisation of the cytoskeleton. Approx 1-2 ml thymidine (100mM) into 20ml FCS medium (T150 flasks) for mammalian cells. |
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By Bubi
on 03-Feb-2009
Pls could i get some protocols for arresting cell cycle in G1 phase and G2/M, |
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By jibe14
on 02-Feb-2009
I would have to syncronize pancreatic cells with horse serum?? I am not sure it will be convenient so if you can send me the protocol as I will do comparison. Thanks in advance |
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By Sreerekha
on 06-Jan-2009
I want to study the effect of some drugs on the different phases of cell cycle. Would you please send me the protocol? |
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By David
on 08-Dec-2008
Hi, |
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By Khushwant
on 07-Dec-2008
I have a doubt regarding synchronization, if the cels are synchronized by a suitable method, may be by serum starvation, then what will be its effect onthe growth of the cells?? |
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By Neha
on 02-Dec-2008
Please send me the synchronization protocol for S phase and G2M phase for cancer cells |
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By Marc Coussens
on 20-Nov-2008
I am doing some expression studies at various cell cycle stages. Can you please send me the protocol? |
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By Ricardo
on 11-Nov-2008
I need to synchronise macrophages. Can I receive the protocol? |
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By ana
on 25-Oct-2008
I would like to have this protocol. I need to synchronise my primary fibroblasts. Thanks |
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By xiaomi
on 22-Oct-2008
I'm interested in cell cycle and cell synchronization. Would you please send me the protocol? Thank you. |
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By Wenying Zhang
on 01-Oct-2008
I am very interested in cell synchronization protocols, Could you send them to me please? |
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By CPhil
on 15-Sep-2008
I need to synchronize cells grown in serum-free media. I can use serum starvation for my other cell line, but not sure of protocol for those grown in serum-free media. I'd really appreciate some protocols. |
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By Artauq
on 04-Sep-2008
I am interested in synchronization and cell cycle. I'd like to recieve a protocol. |
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By Juliana
on 01-Sep-2008
I am needing to perform cell cycle synchronization. Could you please send me the protocol? |
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By hongwei luo
on 28-Aug-2008
i am going to do a research about the expression of my interesting gene involving the cell cycle, so please send me the protocol, thanks! |
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By shuang ma
on 02-Aug-2008
I am going to do synchronization and cell cycle analysis. I would like to receive a protocol. |
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By arunima Biswas
on 24-Jun-2008
Hi, I want my cells (HeLa) to be arrested in G1/S. Please send me the protocol. |
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By lorenzo
on 16-Jun-2008
very good! i want one because i am going to do some studies involving synchronization. |
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By supriti ghosh
on 02-Jun-2008
I'm interested in cell cycle and cell synchronization. Would you please send me the protocol? Thank you. |
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By Shevani
on 27-May-2008
Hi. |
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By Manjula Darshi
on 16-Apr-2008
I am very interested in synchronizing cells and would like to have various protocols. Can you please send me the pdf version of it |
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By mdseay
on 31-Mar-2008
This idea seems promising. I would also like to recieve a pdf containing the protocol. Thanks. |
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By ritu
on 09-Mar-2008
I would like the protocol for cell cynchronization in Hela by serum starvation |
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By priya
on 25-Feb-2008
i am interested in cell syncronization.i'd like to recieve the details about it.........thank u |
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By JR
on 04-Feb-2008
I would like to recieve the protocol in synchronization of cells. |
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By Sunny
on 16-Jan-2008
I'm interested in cell cycle and cell synchronization. Would you please send me the protocol? Thank you. |
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