Technique / Histology / Immunohistochemistry techniques


Problem with nonspecific antibody binding



Problem with nonspecific antibody binding

I am currently developing a protocol for immunofluorescent
detection of the stress proteins hsp70 and cpn60 on cryosectioned Mytilus
mantle tissue. The problem I am having is nonspecific binding of the
secondary antibody (conjugated to fluorescein), leading to strong
false-positive signals. I have tried blocking with bovine serum albumin,
fetal bovine serum, and horse serum, with little or no effect.

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Last update 27-Apr-2004, Rating of 1 votes.

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By Souvik Kar on 22-Mar-2006
Sir,
I am presently working on standardizing the technique,Immunohistochemistry on Paraffin sections.I am getting a lot of non-specific staining by using the substrate Vector Nova Red.Actually I am getting staining in the Control also.
Please suggest me how to remove this non-specificity.
Souvik kar,
IMGENEX India.
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By Nabi on 27-Apr-2004
Dear sir,

I have extrated the total RNA from the xerophytes ,the total RNA looks more viscous .How can i remove the viscous material from my total RNA and It is also contaminated with DNA.Please give me a suggestion how to remove the mucilage and the DNA from the Total RNA.

B.Nabi

Pondicherry UNIVERSITY,

INDIA
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