Technique / Molecular Biology


Molecular Biology Techniques Manual (online 3rd ed)



Molecular Biology Techniques Manual (online 3rd ed)

Excerpts from Molecular Biology Techniques Manual (Third Edition)

Edited by: Vernon E Coyne, M Diane James, Sharon J Reid and Edward P Rybicki

Copyright April 1997, July 1998, May 2000, March 2001, March 2003

Contents:

* Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
* Immunoelectroblotting ("Western" Blotting) of Proteins
* "Lunchbox" Immunoabsorption Techniques
* Affinity Purified Monospecific Antibodies to Gel-Purified Proteins
* Polymerase Chain Reaction?
* PCR PRIMER DESIGN AND REACTION OPTIMISATION
* STANDARD PCR PROTOCOL
* REVERSE TRANSCRIPTION PCR: RNA -> LOTS OF DNA
* CORE SAMPLE PCR
* CALCULATING CONCENTRATIONS FOR PCR
* Detection of Nucleic Acids by Hybridisation

Last update 03-Jul-2007, Rating of 1 votes.

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