Technique / Proteomics and protein biochemistry / Protein Mass Spectrometry

The principles of MS quantitation

The principles of MS quantitation

From IonSource maintained by Andrew Guzzetta.

Table of Contents:

1.Introduction to MS Quantitation
*Total Ion Current (TIC)
* Selected Ion Monitoring (SIM)
* Selected Reaction Monitoring (SRM / MRM)

2.Development of an SRM Transition

3.The Internal Standard

4.PK/LC/MS/MS Reverse Phase Chromatography Method Development

5.Sample Preparation

6.Standards and Standard Curve Preparation
* Standard Curve Creation Procedure
*Example Standard Curve Dilution Scheme
*Running the Standard Curve
*Randomizing Standards and Samples
*Running System Suitability
*The "QCs"

7. Links to PK Resources

Last update 16-Aug-2005, Rating of 20 votes.

Post your message in Protein Mass Spectrometry forum

Write your comment

By mahendra sahu on 05-Dec-2008

i want to know troubleshooting those will come during subject sample analysis means during more than 100 sample analysis
Rating:

Reply

By jyoti wadhwa on 03-Dec-2008

want to know about method development and validation details related to lcms/ms. also want to know selection of buffers as well as solvents for lcms/ms. Thanks

Rating:

Reply

By Dr. Ashok Patel on 13-Sep-2008

MS/MS stands for Triplequad where u can get the mass of the fragment i.e daugher of daughter ion.
Rating:

Reply

By Sunil S. Pokharkar on 13-Sep-2008

I want to know about all the information about LC-MS/MS. such as troubleshooting
Rating:

Reply

By Saurabh on 16-Jun-2008

Lc-ms/ms having triple qadruple,but only two qadruple q1 & q3 using for ion filtration on its m/z ratio and q2 is using for theformation of daughter ion from its parent ion.
Rating:

Reply

By guolin zhang on 18-Feb-2008

i want to know the rule and troubleshooting of LC-MRM
Rating:

Reply

By himanshe on 12-Dec-2007

I wanted to know wat does LCMS/MS stand for. Why is MS repeated.
Rating:

Reply

By yarrasivasekhar on 05-Dec-2007

i want to know impurity profile in LC_MS/MS
Rating:

Reply

By MAGAN VAGHASIYA on 24-Oct-2007

i want to know the troubleshootings related to instruments when we does the validation of blood plasma

Rating:

Reply

By G.Sundhar raja on 22-Jun-2007

i want to know about the principles of MS quantitation
Rating:

Reply

By imranali on 20-Mar-2007

wanna know more about method development
Rating:

Reply

By Surendra Pawar on 22-Jan-2007

I wanted to know How to minimise the time gap between two injections of LC-MS/MS when it attach with Agilant 1200series hplcand also i want to know about method development details and troubleshooting details related to LCMS/MS

Rating:

Reply

By santosh gupta on 15-Jan-2007

i want to know the troubleshootings related to instruments when we does the validation of blood plasma
Rating:

Reply

By Rajesh M shetty on 04-Oct-2006

this kind of notes are really helpful for freshers in this field like me
i would like to know about the criteria to be considered and kept in mind for method devlopment and trouble shooting thereafter
Rating:

Reply

By SENGER (arvind singh senger ) on 28-Apr-2006

i want to know about Qtrap ms system phenomenon and importance in bioanalytical
Rating:

Reply

By arvind singh senger (shekhpur,jaloun) on 23-Mar-2006

want to know about method development troubleshooting details related to LCMS/MS

Rating:

Reply

By senger arvind singh (Sr.research scientist,veedacr on 22-Mar-2006

i want to know about method development details and troubleshooting details related to LCMS/MS & hardware trouble shooting of lcmsms and hplc.

Rating:

Reply

By arvind singh senger on 28-Feb-2006