Golgi apparatus isolation protocol

An classical method for purification of golgi apparatus enriched fraction from leukaemic cells. (A Warley and G M Cook)

Isolation of a Golgi-apparatus-enriched fraction from leukaemic cells A Warley and G M Cook
Biochem J. 1976 May 15; 156(2): 245–251.

Abstract: 1. A Golgi-apparatus-enriched fraction was isolated from acute leukaemic lymphoblasts of AKR mice by using an homogenate stabilized with 1 mM-glutaraldehyde. 2. The isolated fraction, which was shown morphologically to be enriched in dictyosomes, possessed between 44- and 76-fold increase in specific activity, compared with the tumour homogenate, of UDP-galactose-glycoprotein galactosyltransferase and between 3- and10.5-fold increase in relative specific activity of UDP-N-acetygalactosamine-polypeptide N-acetylgalactosaminyltransferase. 3. Plasma membranes isolated from the leukaemic lymphoblasts also possessed glycoprotein galactosyltransferase activity, though in contrast with Golgi-apparatus-enriched material had no detectable polypeptide N-acetygalactosaminyltransferase. 4. The difficulties associated with maintaining the morphological integrity of the Golgi apparatus in subcellular fractionation are discussed.

Last update 21-Nov-2002, Rating of 2 votes.

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	By Josh Ahn on 06-Dec-2005 I am also trying to find a connection between cancer and golgi bodies. How can you use golgi bodies to control or eliminate cancer? One idea I have is to find a molecule that will stop the process of golgi bodies, and eventually, the cell will die. Any suggestions? Rating: Reply

	By Brad Phillips` on 06-Dec-2005 Hey I am a student at Beaverton High School in a Biology II class. We are currently searching for the cure or a control for cancer using the golgi body apparatus. I was wondering if you had found any information on this. Thanks a lot! -Brad Rating: Reply

By Rachel Spisak on 21-Nov-2002 We are a group of highschool students working to find a link between golgi bodies and cancer. We have a biology book that has given us a broad overview of the function of the golgi body. Could you please send us information that would be useful for us to find a cure for cancer using the golgi bodies. Please respond as soon as you can. Thank you for you time. Rating: Reply

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