Technique / Genetics / Cytogenetics analysis

Cytogenetics information site

Write your comment

By Missy on 14-Jul-2008 This is assuming constitutional bloods: Are you setting your flaks flat sided or upright? If flat, try setting upright. Also, swivel the flasks daily while in culture to mix them (don't let them sit for 72 hours). I would suggest using conical tubes, not flasks. Also, try this: 10 ml cultures using 0.6 ml whole blood and 10 ml of this mixture: 100mL RPMI, 11ml FBS, 1ml P/S, 1mg/ml PHA. Rating: n/a Reply

By Olalekan Oguntade on 20-Apr-2008 I am working with fish samples, i found it difficult fixing my samples. I injected my fish with 0.06%Colchicine (1ml/100g BW) 4hrs before crushing the gill epithelium and tissue and kidney. I then placed in 0.75%KCl for 30 minute @ room temp before centrifuge for 10minute at 3000rpm. the sediment was then fixed with glacial acetic acid and methanol (1:3) and then centrifuge before staining in 5% Giemsa solution in 6.8 Phosphate buffer for 30 minutes. Please advise and suggest better protocol. Regards Rating: n/a Reply

By ahmad on 15-Jan-2008 Could someone please advise me about my cytogenetic cultures. I am facing a situation where the blood after settling on the bottom of culture flask assumes a shape of a flake or a viscous clot. I am using standard protocols using 5 ml RPMI-1640 culture medium, 1 ml FBS, 50 micro liter of P/S, 100 mic-liter of l-glutamine and 50 micro liter of phytohaemaglutinin cultured for 72 hours. But the troubleshootin even starts after 12 hr culturing at 37c Rating: Very Good Reply