Technique / Histology / In situ hybridization


50 FISH protocols (Oxford Practical Approach Series)



50 FISH protocols (Oxford Practical Approach Series)

There are 50 protocols in this section:

  • Autoradiography for mRNA detection in mouse embryo tissue sections
       Protocol

  • Chick, mouse, and Xenopus two colour whole mount in
    situ hybridization staining with Fast Red and TrueBlue
       Protocol

  • Culture and harvesting of human metaphase chromosomes
       Protocol

  • Denaturation of DNA in ultrathin sections of Lowicryl K4M embedded material
       Protocol

  • Detection and amplification of FISH signal
       Protocol

  • Detection of multiply spliced HIV-1 tat mRNA by RT-PCR in
    situ hybridization
       Protocol

  • Detection of single-copy HIV-1 DNA by PCR in situ
    hybridization
       Protocol

  • Detection of single-copy HIV-1 DNA using Real-Time (Taqman) PCR in
    situ
       Protocol

  • Developing and staining slides for mRNA detection in mouse embryo tissue sections
       Protocol

  • Direct fluorescence in situ hybridization to Drosophila embryo
       Protocol

  • Double fluorescent in
    situ hybridization of zebrafish or Drosophila embryos    Protocol

  • Enzymatic digestions of Lowicryl ultrathin sections prior to post-embedding
    hybridization    Protocol

  • FISH hybridization and subsequent washes
       Protocol

  • Fixation and pre-treatment of embryos for whole mount
    hybridization    Protocol

  • Fixation and sectioning of cryosections for mRNA detetcion
       Protocol

  • Fixation and storage of mouse embryos for mRNA detection
       Protocol

  • Hybridization of mouse embryo sections for mRNA detection
       Protocol

  • Hybridization of sections, washing, and detection of probe
       Protocol

  • Hybridization solution: composition, heat treatment prior to post-embedding in situ hybridization

       Protocol

  • Immunolocalization by avidin biotinylated enzyme complex staining of zebrafish embryos after in situ hybridization
       Protocol

  • Immunolocalization by avidin biotinylated enzyme complex staining of
    zebrafish embryos after in situ hybridization
       Protocol

  • Immunolocalization by the peroxidase anti-peroxidase (PAP) method after in
    situ hybridization
       Protocol

  • Immunolocalization with a horse-radish peroxidase-conjugated secondary antibody after in situ hybridization
       Protocol

  • Immunophenotyping simultaneously with RT-PCR in situ hybridization, DNA-PCR in situ hybridization,
    and in situ hybridization
       Protocol

  • Labelling of probes for FISH by nick translation
       Protocol

  • PCR in situ hybridization for a representative tissue control (HLA-DQalpha amplification)
       Protocol

  • Post-embedding in situ hybridization and detection of hybrids
       Protocol

  • Post-hybridization washes after mRNA detection in mouse embryo tissue sections
       Protocol

  • Pre-absorption of antibody    Protocol

  • Pre-treatment of cryosections for mRNA detection in tissue sections
       Protocol

  • Pre-treatment of sections before mRNA detection
       Protocol

  • Pre-treatment of sections or cells on slides
       Protocol

  • Preparation of Formvar carbon coated grids for ultrathin sections of Lowicryl embedded cells prior to in situ hybridization

       Protocol

  • Preparation of labelled whole chromosome paints by DOP-PCR
       Protocol

  • Preparation of lymphoid cells and tissue for molecular and immunological applications
       Protocol

  • Preparation of sections for in situ hybridization
       Protocol

  • Sectioning mouse embryos for mRNA detection
       Protocol

  • Sectioning of whole mount hybridized embryos
       Protocol

  • Specific detection of double-stranded DNA sequences in Lowicryl K4M sections by in situ hybridization
       Protocol

  • Subbing of slides for mouse embryo sections for mRNA detection
       Protocol

  • Subbing of slides for mouse embryo sections for mRNA detection
       Protocol

  • Successive steps for Lowicryl K4M embedding and ultrathin sectioning of somatic mammalian cells prior to in situ hybridization
       Protocol

  • Successive steps for specific detection of DNA sequences by post-embedding in situ hybridization
       Protocol

  • Successive steps for specific detection of RNA molecules in Lowicryl K4M sections by in situ hybridization
       Protocol

  • Synthesis of digoxigenin or fluorescein labelled RNA probe
       Protocol

  • Synthesis of single-stranded RNA probe for mRNA detection in tissue sections
       Protocol

  • Treatment of slides prior to FISH
       Protocol

  • Two colour ISH - sequential alkaline phosphatase staining with chromogenic substrates of chick, mouse, and Xenopus embryos
       Protocol

  • Two colour in situ hybridization - sequential alkaline phosphatase staining with chromogenic substrates of zebrafish embryos

       Protocol

  • Whole mount hybridization, washing, and detection of probe (method 1)
       Protocol

  • Whole mount hybridization, washing, and detection of probe (method 2)
       Protocol

  • Zebrafish or Drosophila two colour whole mount in situ hybridization staining with DAB and BCIP/NBT
       Protocol 

    • Last update 10-Mar-2005, Rating of 7 votes.

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    By jay on 09-Jun-2008
    can anyone please provide protocol for RNA FISH in fungal spores.......

    thanking you.
    Rating:

    Reply
    By Reza on 26-Mar-2008
    Could you suggest me a good fluorescence microscope model for PGD.
    Thank you.
    Rating:

    Reply
    By Ahmad on 17-Mar-2008
    Identify the Gender of embryo after embryo collection by 2-3 housr using the FISH, please ifyou can help how he is doing like this and he reults 100% correct with true results.please your help in this stat protocol.
    thanks
    Rating:

    Reply
    By Ahmad on 18-Nov-2007
    I need Multicolor FISH/chromosome painting protocols to use identifying individual chromosomes and anomalies in farm livestock. Also if someone can guide me to secure chromosome specific probes/paints for farm animals!
    Rating:

    Reply
    By Dr. Prasad Karnik on 14-Jan-2007
    On 19/09/2006, I had forwarded a question related to plant FISH. Can I hope of getting an answer? Please send protocol to my e-mail ID.
    Thanks!
    Prasad
    Rating:

    Reply
    By Dr. Prasad Karnik on 19-Sep-2006
    This is very helpful information, put in a convenient format. I congratulate you for that!

    May I request you to provide with FISH protocol/s for plant samples, in general, and GM cotton varieties, in particular, please?

    Thanks you.

    Dr. Prasad Karnik
    Rating:

    Reply
    By samarth on 10-Mar-2005
    This is a excellent book for the protocols related to FISH
    Rating:

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