50 FISH protocols (Oxford Practical Approach Series)
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50 FISH protocols (Oxford Practical Approach Series)
There are 50 protocols in this section:
Autoradiography for mRNA detection in mouse embryo tissue sections
Protocol
Chick, mouse, and Xenopus two colour whole mount in
situ hybridization staining with Fast Red and TrueBlue
Protocol
Culture and harvesting of human metaphase chromosomes
Protocol
Denaturation of DNA in ultrathin sections of Lowicryl K4M embedded material
Protocol
Detection and amplification of FISH signal
Protocol
Detection of multiply spliced HIV-1 tat mRNA by RT-PCR in
situ hybridization
Protocol
Detection of single-copy HIV-1 DNA by PCR in situ
hybridization
Protocol
Detection of single-copy HIV-1 DNA using Real-Time (Taqman) PCR in
situ
Protocol
Developing and staining slides for mRNA detection in mouse embryo tissue sections
Protocol
Direct fluorescence in situ hybridization to Drosophila embryo
Protocol
Double fluorescent in
situ hybridization of zebrafish or Drosophila embryos Protocol
Enzymatic digestions of Lowicryl ultrathin sections prior to post-embedding
hybridization Protocol
FISH hybridization and subsequent washes
Protocol
Fixation and pre-treatment of embryos for whole mount
hybridization Protocol
Fixation and sectioning of cryosections for mRNA detetcion
Protocol
Fixation and storage of mouse embryos for mRNA detection
Protocol
Hybridization of mouse embryo sections for mRNA detection
Protocol
Hybridization of sections, washing, and detection of probe
Protocol
Hybridization solution: composition, heat treatment prior to post-embedding in situ hybridization
Protocol
Immunolocalization by avidin biotinylated enzyme complex staining of zebrafish embryos after in situ hybridization
Protocol
Immunolocalization by avidin biotinylated enzyme complex staining of
zebrafish embryos after in situ hybridization
Protocol
Immunolocalization by the peroxidase anti-peroxidase (PAP) method after in
situ hybridization
Protocol
Immunolocalization with a horse-radish peroxidase-conjugated secondary antibody after in situ hybridization
Protocol
Immunophenotyping simultaneously with RT-PCR in situ hybridization, DNA-PCR in situ hybridization,
and in situ hybridization
Protocol
Labelling of probes for FISH by nick translation
Protocol
PCR in situ hybridization for a representative tissue control (HLA-DQalpha amplification)
Protocol
Post-embedding in situ hybridization and detection of hybrids
Protocol
Post-hybridization washes after mRNA detection in mouse embryo tissue sections
Protocol
Pre-absorption of antibody Protocol
Pre-treatment of cryosections for mRNA detection in tissue sections
Protocol
Pre-treatment of sections before mRNA detection
Protocol
Pre-treatment of sections or cells on slides
Protocol
Preparation of Formvar carbon coated grids for ultrathin sections of Lowicryl embedded cells prior to in situ hybridization
Protocol
Preparation of labelled whole chromosome paints by DOP-PCR
Protocol
Preparation of lymphoid cells and tissue for molecular and immunological applications
Protocol
Preparation of sections for in situ hybridization
Protocol
Sectioning mouse embryos for mRNA detection
Protocol
Sectioning of whole mount hybridized embryos
Protocol
Specific detection of double-stranded DNA sequences in Lowicryl K4M sections by in situ hybridization
Protocol
Subbing of slides for mouse embryo sections for mRNA detection
Protocol
Subbing of slides for mouse embryo sections for mRNA detection
Protocol
Successive steps for Lowicryl K4M embedding and ultrathin sectioning of somatic mammalian cells prior to in situ hybridization
Protocol
Successive steps for specific detection of DNA sequences by post-embedding in situ hybridization
Protocol
Successive steps for specific detection of RNA molecules in Lowicryl K4M sections by in situ hybridization
Protocol
Synthesis of digoxigenin or fluorescein labelled RNA probe
Protocol
Synthesis of single-stranded RNA probe for mRNA detection in tissue sections
Protocol
Treatment of slides prior to FISH
Protocol
Two colour ISH - sequential alkaline phosphatase staining with chromogenic substrates of chick, mouse, and Xenopus embryos
Protocol
Two colour in situ hybridization - sequential alkaline phosphatase staining with chromogenic substrates of zebrafish embryos
Protocol
Whole mount hybridization, washing, and detection of probe (method 1)
Protocol
Whole mount hybridization, washing, and detection of probe (method 2)
Protocol
Zebrafish or Drosophila two colour whole mount in situ hybridization staining with DAB and BCIP/NBT
Protocol
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Last update 10-Mar-2005, Rating Good of 7 votes.
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can anyone please provide protocol for RNA FISH in fungal spores.......
thanking you.
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Could you suggest me a good fluorescence microscope model for PGD.
Thank you.
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Identify the Gender of embryo after embryo collection by 2-3 housr using the FISH, please ifyou can help how he is doing like this and he reults 100% correct with true results.please your help in this stat protocol.
thanks
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I need Multicolor FISH/chromosome painting protocols to use identifying individual chromosomes and anomalies in farm livestock. Also if someone can guide me to secure chromosome specific probes/paints for farm animals!
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On 19/09/2006, I had forwarded a question related to plant FISH. Can I hope of getting an answer? Please send protocol to my e-mail ID.
Thanks!
Prasad
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This is very helpful information, put in a convenient format. I congratulate you for that!
May I request you to provide with FISH protocol/s for plant samples, in general, and GM cotton varieties, in particular, please?
Thanks you.
Dr. Prasad Karnik
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This is a excellent book for the protocols related to FISH
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In Situ HYBRIDIZATION TO TISSUE SECTIONS
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