Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification / Baculovirus expression system
Sf9 Spinner Culture Media Recipe
Sf9 Spinner Culture Media Recipe
Protocol from Bjokerman group at CIT.
Related resource
INSECT CELL CULTURE & BACULOVIRUS INFECTIONS
Baculovirus Protein Expression Protocol

Plaque assay for baculovirus culture

Freezing and recovering Sf9 Cells

Suspension Culture Infections: Low MOI

Suspension Culture Infections: High MOI

Recovering Virus Stocks from Frozen Cell Pellets

Recovering Low-Titer Virus Stocks

Promotions
Related new technical papers
-
Live-imaging of PKC translocation in Sf9 cells and in aplysia sensory neurons. J Vis Exp. 2011;(50)
Induction of intracellular Ca2+ and pH changes in Sf9 insect cells by rhodojaponin-III, a natural botanic insecticide isolated from Rhododendron molle. Molecules. 2011;16(4):3179-96
Multiple factors conferring high radioresistance in insect Sf9 cells. Mutagenesis. 2009 May;24(3):259-69