Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification


Dye-binding protein assay giving very low signals



Dye-binding protein assay giving very low signals

I've eluted some polyclonal antibodies from an antigen column, and
dialysed them into a suitable buffer - the strange thing is that
although the absorbance at 280nm indicates that the proteins are at a
certain, relatively high level (e.g. 1mg/ml), when using the Bradford
assay (the microassay procedure, using the solution from Bio-Rad), one
gets a very low absorbance at 595nm, indicating a concentration about 2
orders of magnitude less (a positive control with BSA works fine).

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Last update 22-Jul-2003, Rating of 0 votes.

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By samir ghsalkar on 22-Jul-2003
dye binding on proteins
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