Immunostaining with Paraffin Sections
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Immunostaining with Paraffin Sections
From Sato's lab at University of Texas Southwestern medical center. Staining procedure for paraffin sections with antibodies to laminin, fibronectin, collagen type IV.
PDF version of the protocol is available on the web page.
Archived page
Last update 26-Jul-2007, Rating Good of 7 votes.
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I need details about immunostaining of parrafin sections of breast tissue with p63 antibody
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I am standardizing IHC and FAT method for Mareks disease, However positive control (MD affected tissue with negative serum) taking brown colouration/ fluorescence. However, control (SPF section) showing negative stain. Guidse me also sene me IHC and FAT protol.
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Suggest me
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how long can the paraffin block be restained on p53 protein by immunohistochemical staining method without alteration of extent and intensity of colour development after preparation of paraffin block?
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What is your protocol for decolorizing when the hematoxylin was left out of the staining process. Thank-you
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I need Immunostaining with Paraffin Sections protocol
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can you please suggest, why the the entire tissue is not staining only part of the tissue is staining during immunosatining with parffin sections. I use antigen retrival method.
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Could you please e-mail me the protocols of immunohistochemistry techniques.
thank you indeed,
firouzeh
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