Meyer-Teruel Collection of Protocols

Some molecular biology and cell biology lab protcols written by Meyer-Teruel at Stanford University.

Content:

Cell culture and Transfection

RBL cell culture

Protocol for freezing down RBL cells

Extracellular Hepes buffer

8-well transfection protocol using BTX cuvette porator

Mouse mast cell line cell culture (c57 cells)

Plating NIH-3T3 cells into 8-well coverslide

Plating and splitting NIH-3T3 fibroblasts

Fugene transfection protocol

Transfecting NIH3T3 cells with Lipofectamine2000

Transfecting hippocampal neurons with Lipofectamine2000

Cleaning incubators

Calibrating incubators

Incubator serial numbers

Molecular Biology

Transformation protocol using heat shock

Pouring agarose gel

Using spectrophometer to quantitate DNA and RNA

Sequencing

Wondo's miniprep

PCR reactions and primer design

Tips for making new construct by PCR

Primer design for Gateway cloning system

Miscellaneous Protocols

Cytomix: 1) stock and 2) transfection buffer

Protocol for transfecting and fixing RBL cells using the BTX 8-well electroporator

Protocol for beta-hexosaminidase secretion assay in RBL cells

Protocol for grinding ESCAT coverslips

Accessing the lab construct database

Flow sorting, 6/8/04

Archived page

Last update 10-Dec-2004, Rating n/a of 0 votes.