Technique / Molecular Biology / Saccharomyces cerevisiae Yeast protocol
Yeast nucleus staining
Yeast nucleus staining
I am currently using DAPI to visualize yeast nuclei, but the signal from
nucleus is often obscured by strong signal from mitochondria. So I am
thinking about using Hoechst 33258 or 33342. Could someone give me some
info what is the difference between these two and how do they compare to
DAPI? And is there any nucleus specific stain at all?
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Last update 15-Nov-2000, Rating of 1 votes.
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By shamere herder
on 26-Nov-2008
could u please send me a answer to my question stating does yeast have a nucleus |
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By oldpost
on 15-Nov-2000
For PI staining, fix cells in 70% ice-cold ethanol for at least an |
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