Cell biology protocols from scienceboard.net

Protocol collections from Science Advisory Board web site.

Content:

9/22/2003 Phenol extraction of DNA samples
9/30/2003 Northern Blots
10/1/2003 Alkaline Lysis Plasmid Miniprep
10/16/2003 Chromosome preparation and Fluorescence in-situ hybridization (FISH)
10/16/2003 Transformation of Listeria monocytogenes
10/17/2003 H-Thymidine Uptake by Cultured Cells
10/18/2003 Intracellular Cytokine Staining
10/18/2003 Matrigel invasion assays
10/18/2003 Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
10/19/2003 Measurement of Incorporation of 35S-Cysteine into Cellular Protein
10/19/2003 Overexpression of Recombinant Proteins in COS-7 Cells
10/20/2003 Polymerase III In Vitro Transcription
10/20/2003 In Vitro Transcription of RNA
10/20/2003 In Vitro Transcription with SP6 Polymerase
10/21/2003 Labeling Oligonucleotides
10/21/2003 Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
10/21/2003 Human Peripheral Blood Mononuclear Cell Preparation
10/21/2003 TransWell Chemotaxis
10/27/2003 G1/S Phase Block for Cell Synchronization
10/27/2003 G2 Phase Block with Hoechst 33342 for Cell Synchronization
10/27/2003 Metaphase Block for Cell Synchronization
10/27/2003 Preparing Chromosome Spreads
11/7/2003 MITOCHONDRIAL DNA ISOLATION
11/7/2003 Green lab protocol for vacuum infiltration transformation of Arabidopsis
11/7/2003 Cell Suspension Culture of Arabidopsis
11/10/2003 Plant Crosse Protocol
11/10/2003 Protocol for determination of seed moisture content
11/13/2003 In vitro growth of seedlings
11/13/2003 Preparation of Solid Tissue for Aspergillus Galactomannan
11/18/2003 Yeast Prep for FACS
11/18/2003 Pheromone Halo Assay
11/18/2003 PEG Preparation of Plasmid DNA
11/19/2003 Rapid PCR Screening of pRSET bacterial colonies
11/21/2003 Isolate physiologically active thylakoid membranes
11/21/2003 Protein Expression Screening Using Plant Tissue Prints On Nitrocellulose
11/21/2003 Isolation of Chloroplasts from Spinach Leaves
11/21/2003 Lyophilizing Cells
11/24/2003 Giemsa for blood cells and parasites
11/24/2003 Leishman's stain for blood cells and parasites
11/24/2003 Acridine Orange Stain
11/24/2003 Gentle lysis of cells in sucrose-containing Mito-Buffer
11/24/2003 Culture of Human Prostatic Carcinoma Cell Lines
11/24/2003 Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB
12/4/2003 ARABIDOPSIS ROOT TRANSFORMATION ASSAYS
12/4/2003 Agrobacterium-mediated Tobacco Transformation
12/4/2003 Rice Bombardment
12/4/2003 PP2C assay
12/4/2003 Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts : Protoplast Isolation
12/4/2003 Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts : PEG Transfection
12/4/2003 Transient Expression Assay Using Maize Mesophyll Protoplasts
12/4/2003 Histochemical GUS assay
12/4/2003 Cysteine proteinase activity assay
12/4/2003 Western blot
12/19/2003 Spermatozoa Chemotaxis assay using Zigmond Chamber.
1/17/2004 Determination of IC50
1/29/2004 G1/S cell synchnronization using double thymidine block
3/16/2004 Wright's staining
3/16/2004 Cell counts
3/16/2004 Sézary Cell Preparation
3/16/2004 Freezing of Cells with Serum
5/3/2004 Culture of Dissociated Sympathetic Neurons
5/3/2004 Culture of Dissociated Cortical Neurons
5/3/2004 Conditioned Medium for Retinal Explant Culture
5/3/2004 Organotypic Culture
5/3/2004 Preparation of a dispersed neuron culture of rat suprachiasmatic nucleus (SCN)
5/3/2004 Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
5/3/2004 Dissociated Retina Immunohistochemistry
5/3/2004 Antigen Retrieval
5/3/2004 PEROXIDASE-ANTI-PEROXIDASE TECHNIQUE FOR THE IDENTIFICATION OF ANTIGENS IN PARAFFIN WAX SECTIONS
5/3/2004 Cytokine-Induced Proliferation of Indicator Cell Lines
5/3/2004 TNF-alpha-Induced Killing of L929 Cell Line
5/3/2004 IFN-gamma Protection from Viral Infection of Cell Lines
5/3/2004 Cytokine ELISPOT Protocol
5/3/2004 Antibody Neutralization of Cytokine-induced Proliferation of Indicator Cell Lines
5/3/2004 Antibody Neutralization of TNFa-Induced Killing of L929 Cell Line
5/3/2004 Antibody Neutralization of IFNg-Protection from Viral Infection of L929 and A549 Cell Lines
5/5/2004 Pediatric Virus-specific Bulk Stimulation Cytotoxic T Lymphocyte (CTL) Protocol
5/5/2004 HUMAN SOLUBLE INTERLEUKIN-2 RECEPTOR "
5/5/2004 DETECTION OF ISOTYPE SPECIFIC IgG ANTIBODIES
5/5/2004 ELISA, Antibody capture
5/5/2004 Sandwich ELISA for SCCA1 and SCCA2
5/5/2004 NEOPTERIN ENZYME IMMUNOASSAY (EIA) TEST PROCEDURE
5/5/2004 Setting up 2 or 3 Colour FACS analysis
5/5/2004 Yeast FACS
5/5/2004 Immunofluorescence Yeast
5/10/2004 Iindirect immunoflourescence for yeast:
5/10/2004 IIF Protocol (modifications for maintaining GFP fluorescence
5/10/2004 PDS1::HA IMMUNOFLUORESCENCE
5/10/2004 Antibody Conjugation
5/10/2004 Staining for Surface Receptors
5/10/2004 Staining for total receptors
5/10/2004 Yeast IF with Methanol/Acetone Dehydration
5/10/2004 Yeast IF without Dehydration
5/10/2004 RESPONDER CELL FREQUENCY ASSAY (RCF): LPA ?Limiting Dilution
5/10/2004 WHOLE BLOOD LYMPHOCYTE PROLIFERATION ASSAY
5/10/2004 Anti-TLR4 Neutralization of LPS-induced Cytokine Production by Human PBMC
5/10/2004 Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Production by Mouse Peritoneal Cells
5/10/2004 Protocol for RBC Lysis of Mouse Spleen
5/15/2004 Metaphase chromosome preparation
5/15/2004 ADH2 Intron 3 RsaI Polymorphism
5/15/2004 HOXB3 TaqI Polymorphism
5/15/2004 PAH MspI Polymorphism
5/15/2004 RET TaqI Polymorphism
5/15/2004 PAH BglII Polymorphism
5/15/2004 COMT BglI Polymorphism
5/15/2004 APOB XbaI Polymorphism
5/15/2004 PAH XmnI Polymorphism
6/7/2004 Cloning cells by limiting dilution
6/29/2004 How to freeze mamalian cells

Last update 12-Aug-2006, Rating Good of 13 votes.