Technique / Immunology / ELISA assay

ELISA protocol

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	By Morteza Izadyar on 18-Aug-2008 If you get no color by mixing conjugate and substrate, one of the conjugate or substrate does not work properly. you con do cross tests by changing source of substrate at one time and source of conjugate another time. Rating: n/a Reply

	By eka on 26-Jun-2008 Hi everybody, I've made my first elisa experiment I got no color reaction. I'm using HRP conjugated sec. antibody and OPD as a substrate. When I mixed just conjugate and substrate didn't get the colour as well. What can be the problem? Rating: n/a Reply

	By paari on 10-Mar-2008 dear friends ? can any one tell me how to calculate p/n ratio in elisa after reading the sample Rating: n/a Reply

	By Aly Kodio on 27-Feb-2008 je suis à la rcherche du protocole ELISA d'anticorps anti-pLDH Rating: Good Reply

	By herve on 21-May-2007 i performed an Elisa test using Biotinylated alkaline phosphatase as detection enzyme and pNPP as substrate,but i obtained always a very high background signal and no linearity depending on the concentration.could one give me some tip please? Thanks herve Rating: n/a Reply

	By LISBETHH on 02-May-2007 deseo saber o conocer comoe suna examen de la prueva de elisa su spartes su funcioen sy como s esabe si est ainfecatdo todo eos Rating: Very Good Reply

	By Dr. B. Deivasigamani on 17-Dec-2006 we can use sheep red blood cells? Rating: Very Good Reply

	By Aashish on 07-Jan-2005 i got good information about initiating the ELISA in my lab Rating: Very Good Reply