Technique / Immunology / Antibody


Protocols for the conjugation of monclonal antibody to fluorochromes



Protocols for the conjugation of monclonal antibody to fluorochromes

In this series of web pages, protocols, notes, and various illustrations are given to aid in the conjugation of proteins--principally monoclonal antibodies--to fluorescent dyes. (Mario Roederer)

Last update 16-May-2008, Rating of 1 votes.

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By wangleicau on 16-May-2008
I am using a functional blocking antibody (mouse anti-human beta 1 integrin) to block the beta 1 integrin in S1 cells (Human mammary epithelial S1 HMT-3522 cells) before I do the immunostaining, but for the immunostaining I have no other choices but use mouse anti-human (NuMA) as primary antibody, and goat anti-mouse as the secondary. I am afraid that the secondary antibody I use will react with the functional blocking antibody. Will it happen? And is there anyone knows whether the functional blocking antibody I use will give me fake fluorescence? Can I wash the functional blocking antibody out?
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