Technique / Immunology / Antibody
Protocols for the conjugation of monclonal antibody to fluorochromes
Protocols for the conjugation of monclonal antibody to fluorochromes
In this series of web pages, protocols, notes, and various illustrations are given to aid in the conjugation of proteins--principally monoclonal antibodies--to fluorescent dyes. (Mario Roederer)
Last update 16-May-2008, Rating of 1 votes.
Post your message in Antibody forum
Write your comment
|
By wangleicau
on 16-May-2008
I am using a functional blocking antibody (mouse anti-human beta 1 integrin) to block the beta 1 integrin in S1 cells (Human mammary epithelial S1 HMT-3522 cells) before I do the immunostaining, but for the immunostaining I have no other choices but use mouse anti-human (NuMA) as primary antibody, and goat anti-mouse as the secondary. I am afraid that the secondary antibody I use will react with the functional blocking antibody. Will it happen? And is there anyone knows whether the functional blocking antibody I use will give me fake fluorescence? Can I wash the functional blocking antibody out? |
Please Login or Register to Post
Related resource
Preparation Of Peptide-KLH Conjugates For Immunization
Ascites in Rabbits?

Preparing Serum from Whole Blood

Antibody search engine

coupling antibodies to protein A/G

Monoclonal Antibody Production

Purification of Monoclonal Antibody from Hybridoma Culture Supernatant

Fusion protocol for monoclonal antibody production

Promotions