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we are working with the purification of the enzyme alkaline protease the crude enzyme shows good activity but once it is precipitated with ammonium sulfate and dialysed with a buffer tris of 50mM conc. it shows a very little activity by couplet method with casein. plz suggest me how do i improve the activity of my specific enzyme. thanku
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je veux dialyser un surnageant contenant ma proteine dans une solution de saccharose ou dans du saccharose en poudre et je veux savoir si la concentration va se traduire par une perte de sel
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I have dialysed 5ml of my protein sample for the last 3 days with about 15 changes of 400 ml each in a dialysis bag of 10000 MW cut off. But the outside water is still showing a higher conductivity( 7.32 microsiemens) than pure water (4.3 microsiemens). Does it mean that it still has some salt?
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