Technique / Proteomics and protein biochemistry / Chromatography HPLC purification


An interactive troubleshooting guide on High Performance Liquid Chromatography



An interactive troubleshooting guide on High Performance Liquid Chromatography

Some covered problems:

1.Peaks
Broad peaks
Ghost peaks
Negative peaks
Peak doubling
Peak fronting
Peak tailing
Spikes
2.Leaks
Leak at column or fittings
Leak at detector
Leak at injection valve
Leak at pump
3.Recovery
Poor sample recovery
4. Sensitivity
Lack of sensitivity
5. Retention
Changing retention times
Decreasing retention times
Increasing retention times
Retention beyond total permeation volume
6. Equilibration
Slow column equilibration time
Varying retention times
7.Baseline
Disturbance at void time
Drifting baseline
Noise
8. Pressure

Last update 23-Jun-2006, Rating of 2 votes.

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By vivek on 01-Oct-2008
t is very useful to know how to maintain the hplc instruments
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By s. balasundaram on 23-Jun-2006
it is very useful to know how to maintain the hplc instruments , the operations in hplc 's to easily detect fault and error identified.
thanking you,
S. Bala.
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