Technique / Proteomics and protein biochemistry / Chromatography HPLC purification

Downloadable HPLC Troubleshooting

Downloadable HPLC Troubleshooting

From MACHEREY-NAGEL Inc.

Content:

chapter 1
broad peaks
fronting
tailing

chapter 2
double peaks
negative peaks
spikes
ghost peaks

chapter 3
lack of sensitivity
poor sample recovery
high back pressure

chapter 4
fluctuating pressure
increasing pressure
decreasing pressure
basisline drift to high absorption
basisline drift to low absorption
ondulating baseline

chapter 5
baseline noise
baseline noise during gradient elution or isocratic proportion
disturbance at dead time
leaks
decreasing retention time
increasing retention time
fluctuating retention time
fluctuating retention time from insufficient equilibration

Last update 14-Dec-2005, Rating of 19 votes.

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By ramesh babu on 16-Jan-2009

for hplc separation what is the relation between pH of buffer and pKa of analyte
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By praveen kumar P on 27-Nov-2008

how to eliminate some problems
noise in baseline
rise in pressure
fluctuation in baseline
disturbance at dead time leaks
decreasing RT
increasing RT
fluctuating RT
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By sivaramprasad on 11-Mar-2008

i got over sound at pump head: what is the problem
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By P.Umamaheswara rao on 20-Feb-2008

first you check the filter is it dip in bottle or not? and another is your filter containing air bubble so sonicate your filter in methanol.
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By Boxfish on 31-Jan-2008

Not able to open this document. Has it been removed. Have problems with baseline in gradient elution. Baseline goes down as buffer B (50% AcN) is increased????
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By pankaj barethiya on 23-Jan-2008