Mouse ES cell culture

ES cell biology techniques

Collections of cell biology techniques for mouse ES cell culture edited by Dr.Gerard Lazo at USDA.

Content:
· MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE
· Routine Culturing of ES Cells
· ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
· MITOMYCIN C TREATMENT OF PMEFs
· GAMMA IRRADIATION OF PMEF'S
· PMEF FEEDER LAYER CONCENTRATION
· ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
· KARYOTYPING ES CELLS
· VASECTOMY OF MALE MICE
· Normaski Optics
· MEDIA FOR EMBRYO CULTURE AND MANIPULATION
· Suppliers
· PREPARATION OF MICROINJECTION PIPETTES
· EMBRYO MANIPULATION PIPETTES (FLUSHERS ETC)
· COLLECTION OF MORULAE and Earlier
· Aggregation of ES cells and eight cell stage embryos
· BLASTOCYST TRANSFER
· Sending sera to testing for mouse pathogens.
· SUPEROVULATION OF MICE
· FLUSHING OUT OF BLASTOCYSTS
· Alizarin Red S (bone) and alcian blue (cartilagestaining of cleared skeletons.
· Routine Splitting and freezing of cells
· SOFT AGAR ASSAY FOR COLONY FORMATION
· VIRUS INFECTION OF FIBROBLASTS
· DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE
· LIPOFECTAMINE FOR TRANSFORMATION OF EUKARYOTIC CELLS
· ELECTROPORATION OF COS-7 CELLS
· Growth and transfection of PC12 cells.
· CELL CULTURE FROM WHOLE MICE EMBRYO (DAY 10 - 11 PC)
· PCR GENOTYPING FROM the EMBRYO YOKE SACK
· b-gal staining of eukaryotic cells in vitro
· Comments on db-cAMP and forskolin
· Comments on Ro 31.8220
· IMMUNOFLUORESENCE ON CHAMBER SLIDES
· PHOTOMICROGRAPHY
· Reagents

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Last update 10-Oct-2005, Rating Very Good of 1 votes.