Technique / Histology / Immunohistochemistry techniques
Immunocytochemistry double staining with rabbit antibody
Immunocytochemistry double staining with rabbit antibody
I wish to stain fixed cells for two different poteins. I have selective
antibodies to each and one which recognises both. Unfortunately, they ae
all raised in rabbit. Is there a simple way of labelling the antibodies
directly, so that I could double-stain, or would omitting a secondary
antibody step lose too much amplification.
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