BD Living Colors FAQ's - Troubleshooting

Troubleshooting and FAQ (BD biosicence):

Q I have trouble detecting GFP variant expression in transiently transfected cells. What can I do?

Q How can I troubleshoot fluorescent protein expression in yeast?

Q How can I troubleshoot no or low signal on Western blots?

Q I selected GFP expressing stable clones that express stable clones and, after culturing, the fluorescence levels have decreased or become variable. What could be the reason for this?

Q I selected stable clones that express GFP and, after culturing for some time, the fluorescence levels have decreased or become variable. What can I do about this?

Q Why do I see aggregation or clumping in cells that express DsRed1?

Q What can I do to decrease aggregation in cells that express DsRed1?

Q I am having trouble selecting stable DsRed1-expressing cells using cloning discs and antibiotic selection. What can I do?

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Last update 09-Jan-2005, Rating n/a of 0 votes.