Technique / Cell Biology / Cell proliferation assay and cell viability assay


MTT cell viability assay



MTT cell viability assay

The MTT assay, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], has been used to test cytotoxicity of reagents and cell viability.

MTT cell proliferation assay (introduction and protocol from ATCC)

MTT cell viability assay (protocol from Daniel Ge)

Recent MTT assay technique papers:

1: Wang X, Ge J, Wang K, Qian J, Zou Y.
Evaluation of MTT assay for measurement of emodin-induced cytotoxicity.
Assay Drug Dev Technol. 2006 Apr;4(2):203-7.

2: Burton JD.
The MTT assay to evaluate chemosensitivity.
Methods Mol Med. 2005;110:69-78.

3: Zhang X, Le Pennec G, Steffen R, Muller WE, Zhang W.
Application of a MTT assay for screening nutritional factors in growth media of
primary sponge cell culture.
Biotechnol Prog. 2004 Jan-Feb;20(1):151-5.

4: Plumb JA.
Cell sensitivity assays: the MTT assay.
Methods Mol Med. 2004;88:165-9.

5: Molinari BL, Tasat DR, Palmieri MA, O'Connor SE, Cabrini RL.
Cell-based quantitative evaluation of the MTT assay.
Anal Quant Cytol Histol. 2003 Oct;25(5):254-62.

Last update 22-Mar-2001, Rating of 27 votes.

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By Ahmed El Sayed on 11-Mar-2009
Hi sir,

I would be appreciated if you can send me the full protocol for MTT assay for cancer cell lines(MKN1)

I plated them in a density of 500.000 cells/55.5 cm2 dish

Regards,

Ahmed
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By bhavin on 11-Mar-2009
Respected Sir,

i m research scholar and i have carried out the MTT assay on mice splenocyte successfully.I have isolated compound. now i want do it on cell line. can you suggest some laboratories in india who can do this assay on commercial basis..
please help..
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By Ranganatha on 16-Feb-2009
Hi
I am planning to do MTT assay experiment, can you please send me complete set of the experiment and protocol
Thank you
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By Amani Soliman on 11-Feb-2009
Hi,
I'm doing MTT cell viability assay for rat RAW264.7. I plated them in a density of 3000 cells/well in a 96 well plate. The aim is to define the treatment dose for an osteoclast differentiation experiment that will go on for 5 days.
I'll appreciate it if you would send me the full protocol for MTT assay for RAW 264.7rat cells.

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By Mohamed Shaheen on 30-Jan-2009
Hi sir,

I would be appreciated if you can send me the full protocol for MTT assay for neonatal rat cardiomyocytes.

I plated them in a density of 200.000 cells/well in a 96 well plate.

Regards,

Mohamed Shaheen
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By Supriya on 29-Jan-2009
sir i m doing MTT assay in C6 cells but and seeding 100000 cells and taking absorbance at 550 with reference to 630 nM but few of the times reading is out of range why??
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By Ethiraj on 24-Jan-2009
Sir,
Am doing cytotoxicity study with some synthesised chalcones, but during the study i faced the problem of soluablity of chalcones in very low concentartion. How can we tackle the problem of soluablity of chalcones and which is the ideal solvent for Chalcones in MTT assay.Please do a help in this matter...
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By saami on 13-Jan-2009
hey if anyone can help me on my project/disseration on buccal cel viability, and the MTT assay , email me, click on ma name saami, and find my email address ,
thank you
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By lav kush tarun on 14-Dec-2008
sir,
i want to know that O.D. depends on the colour in mtt assay. how?
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By indra on 15-Sep-2008
Du kan også bruk colony forming assay for å teste celle levedyktighet.
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By harmanmeet kaur on 02-Sep-2008
please explain the effect of pH on MTT assay
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By zaineb on 20-Aug-2008
dear sir i am zaineb phd student i want detail information about MTT assay for cell viabilty
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By sunil yadav on 08-Jul-2008
Respected Sir,
I am a M.Pharma student doing project. i needs a MTT assay method (protocol) to carry out in-vitro hepatoprotective activity
Plz help and oblige me,
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By Cuihui Zuo on 23-Apr-2008
Sir,
I'm undergraduate pharmacy student currently doing a project on 'Investihation into the neurotoxic effects of Echis carinatus and Daboia russelli snake venoms on selected central nervous system tissues'. We chose to work on the normal cells and tumor cells-lymphoma.
We need to use MTT assay to test the cell viability and Annexin V to test epitose induction and have no idea what are the procedures for these two, the appropriate concentrations of substances to use within the two tests for a size of 30people or more and if any necessary dilution with set conditions.
Would be grateful if you can point me at the right direction.
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By arvind kumar pandey on 10-Apr-2008
dear sir,i am doing work in drde gwalior,i am doing on cell line plz suggest me that what is the concentration of mtt,if i am taking 1ml media.
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By joanne on 25-Mar-2008
dear sir, i am student from Malaysia doing my master project ,
i wonder whether MTT assay is applicable on microalgae cells?

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By aquaplasmid on 01-Feb-2008
Why are you still using MTT, while you have AquaBluer or alamarble etc that is 10x cheaper, simpler, nontoxic, and more reproducible assay available?
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By OMAR on 05-Jan-2008
SIR
IAM STUDENT IN MEDICAL SCHOOL OF AMERICAN UNIVERSITY AND I HAVE ARESEARCH ABOUT THE HUBBLE BUBBLE ( WATER PIPE) AND THEIR EFFECT ON THE LUNG CELL AND IN MY PROCEDURE I HAVE MANY ERRORS TO COMPLETE THIS RESEARCH AND AS YOU KNOW THE RESEARCH NEED THE MTT AND NO AND OTHER FACTORS OF CELL CULTURE AND IF YOU HAVE THE CORRECT PROCEDURE OR CORRECT KNOLEDMENT ABOUT IT PLEASE HELP ME NOW !
THANKS
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By Anil Taneja on 03-Jan-2008
Respected sir,
i am Anil taneja doing my M. pharm project work on nanoparticle of anticancer drug i want to perform MTT assay for my project, so it will be very kind on your part if you please tell me about MTT And Price to perform
Thanks
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By SHUBI on 16-Nov-2007
what is the accuracy and efficiency of MTT assay?
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By Raj solanki on 19-Oct-2007
Sir,
my self an research scholar from india and want to perform MTT assay of the compounds synthesised be me. hence intrested to know the prize and avalibility of chemicals/ kit required. I am also intrested to know whether any lab can do these test for me at an economic rate for me.
thanking you
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By Neelesh kumar on 15-Sep-2007
Dear sir,
This is neelesh kumar doing the M.Pharm (pharmaceutics) from Dept. of Pharmaceutical Sciences Sagar (M.P),India. working with group on drug delivery via the various nanoparticles as carriers and want to do MTT assay so tell me thy price of MTT in Rs. thank sir
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By sachin sakat on 13-Sep-2007
sir, i wish to work on MTT assay.......so can u plz tell me the approximate price of the reagent MTT in Rupees.........Thanx...........
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By santosh nagwani on 16-Jul-2007
Sir
I want to know how to decide the volume of MTT to be added in medium
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By brook on 16-Aug-2006
hello, its better if you sentrifuge your 96-welles plate then remove the media slowly.
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By R kaur on 18-May-2006
What happens with overincubation with MTT reagent, in case formazan crystals are not found within 5 hours incubation.
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By ram chetri on 29-Jul-2005
what is MTT mean
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By bibek on 05-May-2004
I am working with suspension cells and would like to know if the cell washing step, done before addition of MTT can be eliminated without affecting the overall quality of the assay.
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By Flamingrose on 22-Nov-2002
hello, Im a 10th grade biology student looking for a substance that regulates or is regulated by The Cell Cycle please help I have gone through countless research attempting to find it in my teachers text books from her colledge years thank you so much,p.s. she said it was one sertain substance!
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By Jackie LaFleur on 22-Mar-2001
Have you tried searching in "Current Immunology protocols"?
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