Technique / Proteomics and protein biochemistry / Immunoprecipitation


Protein A Sepharose Regeneration



Protein A Sepharose Regeneration

I am using Protein A Sepharose (Pharmacia) for immune precipitations. I
elute immune complexes after binding with laemmli buffer (SDS-PAGE
loading buffer).
Is there anyone who has experience, whether this very expensive material
can be regenerated and reused.

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Last update 16-Mar-2005, Rating of 2 votes.

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By eva on 25-Jul-2007
I did a lot of IPs with proteinA beads but I never reused it and I won't do that because I don't think that I get a complete denaturation at the final step. So, I think that you can still have proteins bound to the beads at the end.
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By rosalia leonardo on 16-Mar-2005
I bought Protein A Sepharose (p6649) (Sigma) for immunoprecipitation but I didn't receive a data sheet, so may anyone help to know how may I use it to precipitate a protein from a total extract, just to have enough protein to determine its N-terminal
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