Technique / Proteomics and protein biochemistry / Immunoprecipitation

Protein A Sepharose Regeneration

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	By eva on 25-Jul-2007 I did a lot of IPs with proteinA beads but I never reused it and I won't do that because I don't think that I get a complete denaturation at the final step. So, I think that you can still have proteins bound to the beads at the end. Rating: n/a Reply

	By rosalia leonardo on 16-Mar-2005 I bought Protein A Sepharose (p6649) (Sigma) for immunoprecipitation but I didn't receive a data sheet, so may anyone help to know how may I use it to precipitate a protein from a total extract, just to have enough protein to determine its N-terminal Rating: Good Reply