Technique / Molecular Biology / RNAi siRNA gene silencing


The siRNA user guide



The siRNA user guide

Review on siRNA application written by Tom Tuschl, Sayda Elbashir, Jens Harborth, and Klaus Weber at Tuschl's Lab, Rockefeller University (revised May 6, 2004).

Last update 22-Jul-2005, Rating of 3 votes.

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By Liat on 15-Dec-2008
Dear Sir
I have established siRNA transfection (60%) in myeloma cell lines. Yet in some of my experiments I have determined significant biological effects without observing protein knockdown. All experiments were conducted in a 72-96h time frame as initially established by viability assays as the maximal effect time point. Can you offer an explanation for this phenomenon?
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By xiaoyun zhao on 21-Jun-2006
Dear sir:
I want to ask you a assistance. Would you like help me?


I engaged in the research of the drug, such as anti-cancer mechanism.


gene=FLT1; gene accession number NM_002019; REGION: 250..4266; Gene ID :2321; organism=Homo sapiens


Thank you for your help in my siRNA Design!


best regards,


xiaoyun zhao



Shen Yang Pharmaceutical University


Dept of Pharmacology


103#,Wenhua Road,Shenhe District


Shenyang 110016


Liaoning Prov.


PR China


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By prathap. k on 22-Jul-2005
Very useful
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