Technique / Proteomics and protein biochemistry / Chromatography HPLC purification


FPLC fast performance liquid chromatography protocol



FPLC fast performance liquid chromatography protocol

The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting.

FPLC introduction and general procedure (Sir William Dunn School of Pathology, Oxford University)

FPLC system manual (AKTA FPLC system)

AKTA FPLC first run and UNICORN program for FPLC

Last update 03-Mar-2005, Rating of 2 votes.

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By vinit shanbhag on 16-Feb-2009
I would like to know which coloumn can give me optimum results for the purification of bromelain extracted from pineapple(this has net negative charge).please help.
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By wenjsh on 03-Mar-2005
Excellent!

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