Technique / Cell Biology / Metabolic labeling proteins
How to show that the proteins are really labeled?
How to show that the proteins are really labeled?
We have fed a radiolabeled polymer to some bacteria and then did an
extraction/fractionation on the culture. One of the fractions is a phenol
solution that supposedly contains proteins.
What we want to know is whether the bacteria incorporated any of the
radiolabel into their proteins. The extract contains 5564 dpm/ml of C-14,
so there is a good chance that there is labeled protein in there. However,
how can we be sure that the counts are coming from protein and not from
something else? Is there ... ...
Last update 02-Jan-2004, Rating of 0 votes.
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By Sibu
on 02-Jun-2004
DNA means Deoxy ribosenucleic acid. This is present in every living organisms. This has four nucleotides viz., Adenine, guanine, thymine and cytosine which are bonded by double or triple hydrogen bonds |
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By naeem
on 02-Jan-2004
What is DNA?Where is it present in the humen being?How can we visualize it? |
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By naeem
on 02-Jan-2004
What is DNA?Where is it present in the humen being?How can we visualize it? |
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