How to show that the proteins are really labeled?
|
How to show that the proteins are really labeled?
We have fed a radiolabeled polymer to some bacteria and then did an
extraction/fractionation on the culture. One of the fractions is a phenol
solution that supposedly contains proteins.
What we want to know is whether the bacteria incorporated any of the
radiolabel into their proteins. The extract contains 5564 dpm/ml of C-14,
so there is a good chance that there is labeled protein in there. However,
how can we be sure that the counts are coming from protein and not from
something else? Is there ... ...
Archived page
Last update 02-Jan-2004, Rating Good of 0 votes.
|
Write your comment
|
DNA means Deoxy ribosenucleic acid. This is present in every living organisms. This has four nucleotides viz., Adenine, guanine, thymine and cytosine which are bonded by double or triple hydrogen bonds Rating: Good
Reply
|
|
What is DNA?Where is it present in the humen being?How can we visualize it?
Rating: Good
Reply
|
|
What is DNA?Where is it present in the humen being?How can we visualize it?
Rating: Good
Reply
|
|
Related resource
Protein metabolic labeling

S-35 and autoradiography

Metabolic Labeling of Cells with 35S

Metabolic labeling of cell proteins with [35S]-met

Metabolic labeling & Immunoprecipitation

A method for efficient isotopic labeling of recombinant proteins

|