Some Proteomic analysis protocols

Protocols from Seattle Proteome Center (SPC), NHLBI Proteomics Center at the Institute for Systems Biology.

Protocols

The following protocols are available in PDF format:
Isotope Coded Affinity Tag Reagents

Acid Cleavable ICAT Labelling Protocol
Orig ICAT (hydrogen/deuterium) Labelling Procedure
Protocol for Making 500 mg of Photo-Beads
Protocol for Labelling with d0d6-GABA Photo-Beads
Isotope Coded Affinity Tag Methods

Profiling ICAT Label Procedure for Budding Yeast Whole Proteome
Stable Isotope Tagging of Affinity Purified Complexes
Tandem Affinity Purification

Mammalian TAP-Tagging Technique
RNAi

RNA interference in THP
Digestion

Trypsin Digestion Procedures
Separation

Cation Exchange Procedure for ICAT Samples
Packing a Reversed-Phase Microcapillary Column

Archived page

Last update 11-Jan-2005, Rating n/a of 0 votes.