Technique / Proteomics and protein biochemistry / Protein electrophoresis
Resolving marginally different proteins on SDS-PAGE gel electrophoresis
Resolving marginally different proteins on SDS-PAGE gel electrophoresis
We occasionally need to resolve hyper- and under-phosphorylated forms of a
protein (pRb) on SDS-PAGE gels for Western blot analysis. Individuals
familiar with these isoforms will know that they differ by an apparent
(maximum of) 6 Kd, and separation is tricky. I'm wondering what those of
you with an interest in the theoretical aspects of SDS-PAGE think about
the use of higher versus ... ...
Last update 26-Apr-2006, Rating of 1 votes.
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By Bhagwat
on 26-Apr-2006
In SDS-PAGE proteins intensity are not uniform, What would be problem? |
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