Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification


Question about Protein A purification of IgG



Question about Protein A purification of IgG

1. What is the molecular basis for the selective binding of IgG by protein
A?
2. What is the difference between Protein A and Protein G in relation to
their Ig class binding specificities?

... ...

Last update 27-Sep-2001, Rating of 0 votes.

Post your message in Protein expression, protein extraction and protein purification forum

Write your comment

By doreen on 27-Sep-2001
hi,



maybe someone can help-I hope so:



I want to do IP and the problem is that my protein (c-myc tagged version and Flag tagged

version as well) binds already to the Sepharosebeads alone (without antibody) !!!! Did

anyone has this problem and knows a solution??? Too much protein or beads???? or is

there a very stringent buffer for more stringent washing procedures?????

thanks

doreen
Rating:

Reply


Please Login or Register to Post