Technique / Proteomics and protein biochemistry / Protein electrophoresis

Heavy and light chains on Coomaasie gel

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By Jim on 17-Nov-2006 Coomassie blue will bind to polypeptides based on charge - generally binding at about the same density for most proteins. What you need to remember is that that band at 53kD actually has much more protein in the same square area of gel as the light chain band. ie there is 106 (2x53) kd worth of peptide in the 53kd area and only about 46kD length of peptide (2x23) in the light chain band. So there is at least twice the protein available for the coomassie blue to bind to in the upper band, thus making that area at least twice as blue. Rating: Very Good Reply