Technique / Molecular Biology / Cloning library construction and screening
Heat Shock Transformation of Chemically Competent Cells
Heat Shock Transformation of Chemically Competent Cells
A short yet useful protocol from Chemicon international.
Last update 27-Nov-2008, Rating of 1 votes.
Post your message in Cloning library construction and screening forum
Write your comment
|
By Agiesh Kumar
on 27-Nov-2008
No colonies were seen after transformation, inspite of using right amount of antibiotic. |
Please Login or Register to Post
Related resource
Manual Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Fo
Some vector sequence and map
Protocols for ET cloning
High throughput recombination cloning in E. coli
Genomic cloning troubleshooting
Subcloning oligos into expression vectors
CLONING PROBLEMS
cDNA library screening with antibody
Promotions