Technique / Immunology / ELISA assay


ELISA question



ELISA question

We have been having a problem with developing an ELISA. Here is the method
we are using:


1) We coat the plate with a goat polyclonal The antibody is in a 50mM
carbonate/bicarbonate buffer, pH=9.5, and the coating is for 2 hrs @ 37C
or overnight @ 4C. We are using EIA/RIA plates from CoStar.


2) After 4 washes in PBS, ... ...

Last update 23-Jan-2002, Rating of 0 votes.

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