Technique / Immunology / ELISA assay
ELISA question
ELISA question
We have been having a problem with developing an ELISA. Here is the method
we are using:
1) We coat the plate with a goat polyclonal The antibody is in a 50mM
carbonate/bicarbonate buffer, pH=9.5, and the coating is for 2 hrs @ 37C
or overnight @ 4C. We are using EIA/RIA plates from CoStar.
2) After 4 washes in PBS, ... ...
Last update 23-Jan-2002, Rating of 0 votes.
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By Joseph Peterson
on 06-Sep-2002
We are non profit and would like to open a lab in Ethiopia. Price list for new and old lab equipment is my interest. Thanks JP |
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By shiyongqi
on 30-May-2002
ÎÒ¡ã´ÕÕ¡¶Öй¨²É¨²ÎïÖÆÆ¡¤¼¨¬¶¡§¹æ³Ì¡¡¤ÓüÐÐÄ¡¤¡§¡Áö¡¡ã¾¨²Ì嵡ã¡ã¡Á²ÐÁôÁ¿²â¶¡§¡¡À¡£µ«½¨¢¹û¡ÁÜûÓÐÌݶȣ¬ÎÒÊÔÓõ¡ÁÎïÒº¼ÓÁ¨°Ë¨¢ÖÕÖ¹Òº¹Û²¨¬ÑÕÉ«Ó뽨¢¹ûÑÕÉ«´¨®ÖÂϨ¤Í¬£¬ÎÒÏë¿ÉÄÜÊǵÚÒ»²½¿¹ÌåûÓС㨹¡À»ÉÏ¡£Çë´¨®¼Ò¡ãïæ½â´ðһϡ££¡§Ë¨´ÓÃÊÔ¼Á¼¡ã¿¹Ì徨´Óë¹æ³ÌÉÏÒ»Ö£¬Ò¨°ÎÒÊÇÐÂÊÖ£¬¿ÉÄܺö¨¤µØ¡¤½´æÔÚÎÊÌ⣬¨¦¡¤³´¨®¼ÒϨºÏ¸Ö¸µ¼£¬ÌØ¡ÀðÊÇÈçºÎ¡ã¨¹¡À»£¬ÈçºÎÏ´µÓ£©¡£¡¤Ç³£¸Ðл¡£ |
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By oasis
on 23-Jan-2002
hrp |
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