Technique / Immunology / ELISA assay

Ceramide ELISA

Ceramide ELISA

I have coated ELISA plates with Ceramide - 1000 - 100 - 10 and 0 ng/ml (100
mcl/well) using 2 coating buffres (ph 9.6 and 7.2).

Hereafter we added a mouse anti ceramide antibody and a biotinylated rabbit
anti mouse antibody, TMB etc., with no result. All wells gave the same as
the "0" = bagground.

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Last update 04-Sep-2003, Rating of 2 votes.

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By mrityunjay on 18-Sep-2008

i m also working on ceramide..
bt u can not coat it on simple polystyerene plates..
u hv to try some other coating,,, before going for ceramide coating.....

all the best...
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