Technique / Proteomics and protein biochemistry / Immunoprecipitation

Immunoprecipitation protocol from Sigma

Immunoprecipitation protocol from Sigma

Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. Immunoprecipitation followed by SDS-PAGE and immunoblotting, is routinely used in a variety of applications: to determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins. The IP technique also enables the detection of rare proteins which otherwise would be difficult to detect since they can be concentrated up to 10,000-fold by immunoprecipitation (Sigma Aldrich).

Last update 18-Mar-2005, Rating of 1 votes.