Technique / Molecular Biology / Gene targeting / Inducible tetracycline cre-loxP system
Tetracycline-inducible expression systems with reduced basal activity in mammalian cells
Tetracycline-inducible expression systems with reduced basal activity in mammalian cells
Free accessible original paper from pubmedcenter.
Forster K., et al. Nucleic Acids Res. 1999 Jan 15;27(2):708-10
We describe a modification of the tetracycline-inducible eukaryotic gene expression system with decreased basal levels of expression in HeLa cells. It employs the tetracycline-inducible transactivator and a tetracycline-regulated repressor fusion acting on the same promoter. To avoid heterodimerization or competition for the same DNA site, each was provided with different DNA recognition and/or protein dimerization specificities. We achieved active silencing in the uninduced state resulting in approximately 6-fold reduced levels of basal transcription and several hundred-fold activation of gene expression upon addition of tetracycline.
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