Technique / Proteomics and protein biochemistry / Immunoprecipitation


co-immunoprecipitation question: starting concentration



co-immunoprecipitation question: starting concentration

What is a good starting concentration of each protein to have? What volume? I know that my proteins are tight-binding, so dissociation should not be a big problem. (post from NWFSC forum).

Last update 15-Jun-2007, Rating of 2 votes.

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By Kishan Chudasama on 27-Feb-2009
Hi there! I have the same concentration in my nuclear samples, but during IP I reduce the salt concentration to around 100mM.
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By Alvaro Morales on 15-Jun-2007
Hi! I need to do a Co-IP and my buffer have a 420mM NaCl. I would like to know if this concentration is to higth and may disociate the protein-protein interactions
Thanks
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