Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification


Recombinant protein expression and purification flowchart



Recombinant protein expression and purification flowchart

The Flowchart will help you to choose:

* the right domain
* the right expression system
* how to optimize the expression levels of the target protein
* the right method for protein extraction and clarification
* the purification scheme
* the correct way to store the purified protein

The Flowchart is linked to an extensive list of protocols for the different steps in the process. (David Drechsel, Arie Geerlof, Ario de Marco, Gunter Stier, Stephen Weeks, and Hans van der Zandt. EMBL Heidelberg).

Last update 04-Oct-2005, Rating of 8 votes.

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By Cheryl on 22-Jan-2009
Please help me with opening the flow chart. I will be working with different expression systems
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By Dilip on 07-May-2008
I am also working on recombinant protein expression and purification but i am unable to open
this flowchartplz help me out
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By Johan on 06-Nov-2007
About to chose a expression system for membrane proteins, this site gave me some good pointers that I would have had a hard time revealing from articles, although non-membrane proteins were more throughly handled.
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By Dr. isfaq on 09-Sep-2007
Iam going to work on recombinant protein
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By M. Sajjad on 18-May-2006
Hi, It is an excellent effort to help new researchers. Really liked it.
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By Sunitha on 12-May-2006
Purification Hi-tagged recombinant protein cloned in pRSET expression having T7 promoter
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By shabbir on 05-Nov-2005
i am woking on recominant proteins expressed in mammalian cell lines
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By aftab on 04-Oct-2005
there is not a single thing in the world who claim to be perfect, so you should always look forward to make the site more effective and knowledgeable especially for new researchers
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