Technique / Molecular Biology / DNA analysis techniques
Whats going on with phenol?
Whats going on with phenol?
Recently the mini-preps I've done using alkaline lysis and phenol extraction
have been giving extremely high OD's, making them look brilliant, until I
check by restriction digest and agarose gel, to find there's very little
plasmid DNA actually in there. MPs prepared by Qiagen columns worked fine,
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