Fast PCR primer design and sequencing software

Fast PCR software has specific, ready-to-use templates for many PCR and sequencing applications: (Dr. Alan H. Schulman Dr. Ruslan Kalendar, University of Helsinki)

The software can be downloaded from the web page. 

* Standard, inverse and long PCR - Locates optimal primers for PCR, hybridisation, or sequencing.
* Multiplex/nested primers - Prints searched and selected primers over the sequence symbolically and selected primers in tabular format along with their cross-dimers.
* Degenerate PCR directly on amino acid sequence.
* In Silico PCR - Prediction probable PCR products and search of position primer with mismatches.
* Manual Search- Allows researcher to set specific primer properties and locates all primers with these properties.
* Secondary structures - Analyses primers for self-dimers, cross-dimers with alignment and with the melting temperature calculation.
* False priming - Check primers for false priming with alignment and with the melting temperature calculation.
* Analyse existing primers - Paste in any primer sequence for analysis, check for its binding sites on sequence (Powerful Search tool and "in silico PCR").
* Primer quality - Unique way for determination of priming efficiency and PCR.
* Comprehensive primer report - Prints comprehensive report for pairs and individual primers.
* Segment-to-Segment sequence alignment. Minority consensus - generates consensus sequence using degenerate bases.

File Format Support - FASTA, text file, "Excel Sheet", load or paste from clipboard.

Download address last updated by Ruslan Kalendar at May, 30, 2005.

Last update 02-Mar-2005, Rating of 7 votes.

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	By Ruslan Kalendar on 03-Dec-2008 Thank you all, for good opinions of FastPCR and I will improve software continual. Rating: Reply

	By sukhdeep on 22-Aug-2008 very nice and user friendly Rating: Reply

	By neelam sehrawat on 17-Apr-2008 I am using this program for sequence alignment, i found it great and faster than any other software. Rating: Reply

	By kumar on 17-Feb-2007 Its the ultimate machine for PCR primer design which I have ever found. The primers generated by this tool works really well in practical situations. Rating: Reply

	By radhika on 30-Jan-2007 i want to use this programme because of its fast results which saves our time Rating: Reply

	By Chee How Teo on 04-May-2006 I have been using this program for quite a long time. I found it very user-friendly and most of the primer generated by this program work very well in my research. Rating: Reply

	By Rebecca on 02-Mar-2005 It ROXXX! Seriously, it's a great program. Rating: Reply

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