Technique / Molecular Biology / PCR / PCR primer design
PCR primer design and reaction optimization
PCR primer design and reaction optimization
From Molecular Biology Techniques Manual edited by Vernon E Coyne et al. (Written by
Ed Rybicki, University of Cape Town)
Contents
* Factors Affecting the PCR
* Denaturing Temperature and Time
* Annealing Temperature and Primer Design
* Primer Length
* Degenerate Primers
* Elongation Temperature and Time
* Reaction Buffer
* Cycle Number
* Nested Primer PCR
* Labelling of PCR products with digoxygenin-11-dUTP
* Helix Destabilisers / Additives
* Useful Universal cDNA PCR Primer
* A simple set of rules for primer sequence design
* REFERENCES
Last update 12-Apr-2005, Rating of 1 votes.
