Technique / Molecular Biology / DNA analysis techniques


EtOH ppt of DNA, whats wrong with this strategy?



EtOH ppt of DNA, whats wrong with this strategy?

There's got to be a reason for adding NaOAc to the aqueous DNA solution
BEFORE adding the EtOH. Is this an absolute necessity?

I'm doing >1000X precipitations/day of PCR products in 96 well plates, ... ...

Last update 08-Feb-2007, Rating of 1 votes.

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By Xu on 08-Feb-2007
I think the salt has the co-function in the DNA ppt, and the salt that can not dissolute in the EtOH can make the pellet bigger and easier to see.
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