Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification / Baculovirus expression system
Freezing and recovering Sf9 Cells
Freezing and recovering Sf9 Cells
Sf9 cells can be frozen in a manner quite similar to methods used to preserve mammalian cells. This involves putting them in a suitable medium, including 7.5-10% DMSO, and a controlled freezing rate. (Dr. Jim Litts, baculovirus.com)
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